《植物生理学报》 2015, 51(8): 1339-1346

基于叶绿体片段序列的杨属系统发育关系分析

员涛^1,2, 李佳蔓^1,2, 周安佩^1,2, 颜璐茜^1,2, 纵丹^1,2, 李旦³, 何承忠^1,2,4,*

西南林业大学¹云南省高校林木遗传改良与繁育重点实验室, ²西南地区生物多样性保育国家林业局重点实验室, ³云南生物多样性研究院, ⁴西南山地森林资源保育与利用省部共建教育部重点实验室, 昆明650224

通信作者：何承忠；E-mail: hcz70@163.com；Tel: 0871-63863213

摘　要：

以旱柳为外类群, 对杨属5个派32个种及杂种63份个体叶绿体DNA (cpDNA)的rbcL-a、psbI-psbK、psbA-trnH、trnL-F序列片段进行系统发育分析。结果表明, 4个片段联合后的序列长为2•416 bp, G+C含量为34.1%, 含32个信息位点数。最大简约法(MP)和最大似然法(ML)构建的系统进化树的树形一致, 白杨派和大叶杨派能各自聚类为单独分支, 而青杨派树种被聚分为2组, 显示出多系起源的特征。其中, 三脉青杨、青杨、大青杨、辽杨为第1组, 位于系统树的基部, 与胡杨形成姐妹种关系, 其余青杨派树种为第2组, 与大叶杨亲缘关系较近。黑杨派中的钻天杨和北京杨与同派其他树种差异明显, 但与白杨派树种具有同源性, MP和ML的支持率分别为95%和91%, 其余黑杨派树种高度同源, 以100%的MP和ML支持率呈姐妹群。该研究结果为杨属树种的起源与系统进化关系提供了分子水平的证据。

关键词：杨属; 叶绿体DNA; 片段序列; 系统发育

收稿：2015-03-01   修定：2015-07-13

资助：国家林业公益性行业科研专项(201104076)、国家自然科学基金(31360184、31460205)、云南省中青年学术与技术带头人后备人才培养基金(2012HB021)。

Analysis of Phylogenetic Relationship of Populus Based on Sequence Data of Chloroplast Regions

YUN Tao^1,2, LI Jia-Man^1,2, ZHOU An-Pei^1,2, YAN Lu-Xi^1,2, ZONG Dan^1,2, LI Dan³, HE Cheng-Zhong^1,2,4,*

¹Key Laboratory for Forest Genetic and Tree Improvement & Propagation in Universities of Yunnan Province, ²Key Laboratory of Biodiversity Conservation in Southwest China, State Forestry Administration, ³Yunnan Academy of Biodiversity, ⁴Key Laboratory for Forest Resources Conservation and Use in the Southwest Mountains of China, Ministry of Education, Southwest Forestry University, Kunming 650224, China

Corresponding author: HE Cheng-Zhong; E-mail: hcz70@163.com; Tel: 0871-63863213

Abstract:

Using Salix matsudana as an outgroup, the plastid genomic regions, namely, rbcL-a, psbI-psbK, psbA-trnH and trnL-F, were used to investigate the phylogenetic relationship of 63 Populus individuals representing 32 species of 5 sections. Our analysis showed that the total length of four chloroplast regions was 2•416 bp containing 34.1% G+C and 32 informative sites. The consistent phylogenetic tree was presented by maximum parsimony (MP) and maximum likelihood (ML). Section Leuce formed a separate clade, like section Leucoides, whereas section Tacamahaca was divided into two groups, revealing a characteristic of polyphyletic origins. One group contained P. trinervis, P. cathayana, P. ussuriensis and P. maximowiczii located at the base of the tree with sister relationship to P. euphratica, while another group contained other species in section Tacamahaca together with P. lasiocarpa. Belonged to section Aigeiros, P. nigra var. italica and P. beijingensis were clearly separated from their consectional species and grouped with section Leuce with 95% bootstrap supported by MP and 91% bootstrap supported by ML, while remanent species in section Aigeiros exhibited sister relationship with 100% bootstrap supported by both MP and ML. These results provide molecular evidence for the origin and phylogenetic relationship of species in genus Populus.

Key words: Populus; chloroplast DNA; region sequence; phylogeny